Eastern Michigan University
direct edit

Dr. Anne Casper

Associate Professor

401S Mark Jefferson Science Complex

734.487.0212

anne.casper@emich.edu

Education

B.S., University of Nebraska - Lincoln, 1999

M.S., University of Michigan, 2002

Ph.D., University of Michigan, 2005

Interests and Expertise

Abnormal cell growth in cancer cells results from genetic changes, such as amplifications, deletions, and mutations, that alter gene function. These genetic changes can be stimulated by stress during replication, the process of copying a cell's DNA. Replication stress, in which the copying process is slowed or stalled, causes breaks at particular hotspots known as "fragile sites," and many tumors have genetic changes at fragile sites. We use the yeast model system to investigate the fidelity of repair processes at fragile sites, analyze the contribution of fragile site instability to amplifications and deletions, and test hypotheses about why fragile sites are unstable during replication stress. The results of our studies will help us understand how and why genetic changes arise in tumor cells. For the most up-to-date information about my lab and the students working with me, please see my lab website: http://people.emich.edu/acasper2/

Publications

†denotes M.S. student author

*denotes undergraduate author

Rosen DM†, Younkin EM, Miller SD*, and Casper AM. Fragile Site Instability in Saccharomyces cerevisiae Causes Loss of Heterozygosity by Mitotic Crossovers and Break-Induced Replication.  PLoS Genet ics (2013) 9(9):e1003817. doi: 10.1371/journal.pgen.1003817.

Casper AM, Rosen DM†, and Rajula KD†.  Sites of genetic instability in mitosis and cancer. Annals of the New York Academy of Sciences (2012) 1267:24-30.  [conference issue]

Casper AM, Greenwell PW, Tang W, and Petes TD.  Chromosome aberrations resulting from double-strand DNA breaks at a naturally-occurring yeast fragile site composed of inverted Ty elements are independent of Mre11p and Sae2p. Genetics (2009) 183: 423-39.

Casper AM. Colon Cancer: A Case of Genetic Bad Luck? Web-based Case Study Collection of the National Center for Case Study Teaching in Science at the State University of New York at Buffalo (2008).

Casper AM, Mieczkowski PA, Gawel M and Petes TD. Low Levels of DNA Polymerase Alpha Induce Mitotic and Meiotic Instability in the Ribosomal DNA Gene Cluster of Saccharomyces cerevisiae. PLoS Genetics (2008) 4(6): e1000105 doi:10.1371/journal.pgen.1000105

Casper AM, Arlt MF, and Glover TW. "Common Fragile Sites: Cytogenetics and Cell Cycle Checkpoints." Fragile Sites: New discoveries and changing perspectives. Eds. Isabel Arrieta, Olga Penagarikano, Mercedes Telez. Nova Science Publishers Inc., Hauppauge, NY (2007), pp. 47-66.

Miller CT, Lin L, Casper AM, Lim J, Thomas DG, Orringer MB, Chang AC, Chambers AF, Giordano TJ, Glover TW and Beer DG. Genomic amplification of MET with boundaries within fragile site FRA7G and upregulation of MET pathways in esophageal adenocarcinoma. Oncogene (2006) 25:409-18.

Glover TW, Arlt MF, Casper AM and Durkin SG. Mechanisms of common fragile site instability. Human Molecular Genetics (2005) 14 (Supp. 2):R197-R205.

Casper AM, Durkin SG, Arlt MF and Glover TW. Chromosomal Instability at Common Fragile Sites in Seckel Syndrome. The American Journal of Human Genetics (2004) 75:654-60.

Arlt MF, Xu B, Durkin SG, Casper AM, Kastan MB and Glover TW. The BRCA1-dependent G2/M checkpoint pathway is crucial to common fragile site maintenance. Molecular and Cellular Biology (2004) 24:6701-6709. Arlt MF,

Casper AM and Glover TW. Common Fragile Sites. Cytogenetic and Genome Research (2003) 100:92-100. [invited review]

Casper AM, Nghiem P, Arlt MF and Glover TW. ATR Regulates Fragile Site Stability. Cell (2002) 111:779-789.

Courses Taught

BIO 110 Introductory Biology I
BIO 305 Cell & Molecular Biology

Other

Lab website: http://people.emich.edu/acasper2/

The Biology Department is part of the College of Arts & Sciences, 214 Pray-Harrold