Eastern Michigan University
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Jeffrey Guthrie, Ph.D.

Associate Professor

Dr. J in the lab 501H Mark Jefferson Bldg




B.S., Thompson Rivers University, Kamloops, British Columbia, Canada, 1999
Ph.D., Carleton University, Ottawa, Ontario, Canada, 2004

Bioanalytical Chemistry

Interests and Expertise

My current research interests are in environmental bioanalytical/analytical chemistry. My main project is in the development of novel biosensors that use synthetic DNA fragments as probes for the detection of environmental contaminants in aquatic systems. My secondary projects are  collaborative  efforts to determine the anticancer properties of antimicrobial peptide analogues in human cancer cells.


R. Muterspaugh, D. Price, D. Esckilsen, S. McEachern, J.W. Guthrie, D.L. Heyl, H.G. Evans, "Interaction of Insulin-Like Growth Factor-Binding protein 3 With Hyaluronan and Its Regulation by Humanin and CD44Biochemistry201857 (39), pp 5726–5737

H.G. Evans, J.W. Guthrie, M. Jujjavarapu, N. Hendrickson, A. Eitel, Y. Park, J. Garvey, R Newman, D. Esckilsen, and D.L. Heyl, “D-Amino Acid Analogues of the Antimicrobial Peptide CDT Exhibit Anti-Cancer Properties in A549, a Human Lung Adenocarcinoma Cell Line” Protein Pept. Lett., 2017, 24, 590-598

J.W. Guthrie, R.T. Limmer, E.A. Brooks, C.C. Wisnewski, N.D. Loggins-Davis, A. Bouzid, Simultaneous Detection of Ultraviolet B-Induced DNA Damage Using Capillary Electrophoresis with Laser-Induced Fluorescence, Anal. Chim. Acta, 2015, 853, 676-681.

J.W. Guthrie, 3.01 - General Considerations when Dealing with Biological Fluid Samples, In: Editor-in-Chief: Janusz Pawliszyn, Editor(s)-in-Chief, Comprehensive Sampling and Sample Preparation, Academic Press, Oxford, 2012, Pages 1-19

C.G. Weisener, J.W. Guthrie, C. Smeaton, D. Paktunc, J. Fryer, "Effects of Ca-Fe-As coatings on microbial leaching of metals in arsenic bearing mine waste" Journal of Geochemical Exploration, 2011, 110, 23-30

J.W. Guthrie, X.C Le “Aptamers in affinity separations: capillary electrophoresis” Feb 2009, Springer, Chapter 9, Functional Nucleic Acids for Sensing and Other Analytical Applications, 252-270

J.W. Guthrie, J. Lee, M. Weinfeld, and X.C. Le, “Mechanistic investigation of the formation of Benzo[a]pyrene Induced DNA Adducts in the Presence of Arsenic” Manuscript in preparation.

A.A. Goulko, Q. Zhao, J.W. Guthrie, H. Zou, and X.C. Le, “Fluorescence Polarization: Recent Bioanalytical Applications, Pitfalls, and Future Trends” Springer Series on Fluorescence, 2008, 5, 303-322

J.W. Guthrie, J-H Ryu, X.C. Le, and L. Weibe, “Characterization of a Cyclodextrin-Oligonucleotide Complex by Capillary Electrophoresis Using Laser-Induced Fluorescence” J. Pharm. Pharm. Sci., 2007 10(2), 246-255.

J.W. Guthrie, C.L.A. Hamula, H. Zhang, X.C. Le and X-F. Li “Selection and Analytical Applications of Aptamers” TrAC, 2006, 25, 681-691. (Co-first author)

J.W. Guthrie, C.L.A. Hamula, H. Zhang, and X.C. Le, “Assays for Cytokines Using Aptamers” Methods, 2006, 38(4), 324-330.

H. Fu, J.W. Guthrie, X.C. Le, “Study of Binding Stoichiometries of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase by Capillary Electrophoresis and Laser-Induced Fluorescence Polarization Using Aptamers as Probes” Electrophoresis, 2006, 27, 433-441.

C.A. Murimboh, J. Deng, J.D. Murimboh, J.W. Guthrie, C.L. Chakrabarti and D.S. Lean, “An Electrochemical Study of the Interactions between Trace Metals and Humic Substances in Freshwaters by Anodic Stripping Voltammetry with a Thin Mercury Film Rotating Disk Electrode” Electroanalysis, 2005, 17, 1977-1984.

J.W. Guthrie, J. Murimboh, N.M. Hassan, M.S.A. Salam, C.L. Chakrabarti, and D.C. Grégoire, “Speciation of Ni2+, Cu2+, Zn2+, and Cd2+ in some Metal-Impacted Lake Waters: A Comparison of Experimental Data with Predictions from the Windermere Humic Aqueous Models (WHAM) V and VI” Anal. Chim. Acta, 2005, 528, 205-218.

J.W. Guthrie, R. Mandal, M.S.A. Salam, N.M. Hassan, J. Murimboh, C.L. Chakrabarti, M.H. Back, and D.C. Grégoire, “Kinetic Studies of Nickel Speciation in Model Solutions of a Well-Characterized Humic Acid using the Competitive Ligand Exchange Method” Anal. Chim. Acta, 2003, 480, 157-169.


Professional Affiliation

American Chemical Society
Canadian Society for Chemistry

Courses Taught

General Chemistry I Laboratory (122)
General Chemistry II Laboratory (124)
General Chemistry II (123)
Introduction to Analytical Chemistry (283/284)
Fundamentals of Chemical Instrumentation (383)
Biochemistry (453)
Current Topics in Environmental Science and Society (ENVI 305)

The Department of Chemistry is part of the College of Arts & Sciences, 214 Pray-Harrold