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John Carbone

Associate Professor

John Carbone photo 312 Marshall

734.487.3303

jcarbon2(at)emich(dot)edu

Education

PhD Nutritional Sciences - University of Connecticut

BS Biochemistry - Union College

Teaching Interests

Dr. Carbone's research focuses on the role of diet and exercise in maintaining skeletal muscle integrity. Specifically, he's interested in how energy balance and dietary modifications affect the activity of the ubiquitin proteasome pathway of muscle protein breakdown.

Selected Presentations and/or Publications

Book Chapter

Carbone JW, Margolis LM, Pasiakos SM. Cellular and Molecular Mechanisms of Protein Synthesis Among Tissues. In: Dardevet D, editor. The Molecular Nutrition of Amino Acids and Proteins. Boston: Academic Press - Elsevier; 2016. pp. 39-47.

Manuscripts

Carbone JW and Pasiakos SM. Dietary Protein and Muscle Mass: Translating Science to Application and Health Benefit. Nutrients. 2019; 11(5) pii: E1136.

Carbone JW, McClung JP, Pasiakos SM. Recent advances in the characterization of skeletal muscle and whole-body protein responses to dietary protein and exercise during negative energy balance. Adv Nutr. 2019; 10(1):70-79.

Margolis LM, Berryman CE, Murphy NE, Carrigan CT, Young AJ, Carbone JW, Pasiakos SM. PI3K-AKT-FOXO1 signaling is targeted by skeletal muscle microRNA to suppress proteolytic gene expression in response to carbohydrate ingestion during aerobic exercise. Physiol Rep. 2018; 6(23):e13931.

Young AJ, Berryman CE, Kenefick RW, Derosier AN, Margolis LM, Wilson MA, Carrigan CT, Murphy NE, Carbone JW, Rood JC, Pasiakos SM. Altitude acclimatization alleviates the hypoxia-induced suppression of exogenous glucose oxidation during steady-state aerobic exercise. Front Physiol. 2018; 9:830.

Pasiakos SM, Berryman CE, Carbone JW, Murphy NE, Carrigan CT, Bamman MM, Ferrando AA, Young AJ, Margolis LM. Muscle Fn14 gene expression is associated with fat-free mass retention during energy deficit at high altitude. Physiol Rep. 2018; 6(14):e13801. 

Margolis LM, Carbone JW, Berryman CE, Carrigan CT, Murphy NE, Ferrando AA, Young AJ, Pasiakos SM. Severe energy deficit at high-altitude inhibits skeletal muscle mTORC1-mediated anabolic signaling without increased ubiquitin proteasome activity. FASEB J. 2018 Jun 7 [epub ahead of print]

Karl JP, Cole RE, Berryman CE, Finlayson GS, Radcliffe PN, Kominsky MT, Murphy NE, Margolis LM, Carbone JW, Rood JC, Young AJ, Pasiakos SM. Appetite suppression and altered food preferences coincide with changes in appetite-mediating hormones during energy deficit at high altitude, but are not affected by protein intake. High Alt Med Biol. 2018; 19(2):156-169.

Berryman CE, Young AJ, Karl JP, Kenefick RW, Margolis LM, Cole RE, Carbone JW, Lieberman HR, Kim I-Y, Ferrando AA, Pasiakos SM. Severe negative energy balance during 21 d at high altitude decreases fat-free mass regardless of dietary protein intake: a randomized controlled trial. FASEB J. 2018; 32(2):894-905.

Pasiakos SM, Berryman CE, Carrigan CT, Young AJ, Carbone JW. Muscle Protein Turnover and the Molecular Regulation of Muscle Mass during Hypoxia. Med Sci Sports Exerc. 2017; 49(7):1340-1350.

Pastore RL, Brooks JT, Carbone JW. Paleolithic nutrition improves plasma lipid concentrations of hypercholesterolemic adults to a greater extent than traditional heart-healthy dietary recommendations. Nutr Res. 2015; 35(6):474-9.

Pasiakos SM and Carbone JW. Assessment of skeletal muscle proteolysis and the regulatory response to nutrition and exercise. IUBMB Life. 2014; 66(7):478-84.

Carbone JW, Pasiakos SM, Vislocky LM, Anderson JM, Rodriguez NR. Effects of short-term energy deficit on muscle protein breakdown and intramuscular proteolysis in normal-weight, young adults. Appl Physiol Nutr Metab. 2014; 39(8):960-8.

Carbone JW, Margolis LM, McClung JP, Cao JJ, Murphy NE, Sauter ER, Combs GF, Young AJ, Pasiakos SM. Effects of energy deficit, dietary protein, and feeding on intracellular regulators of skeletal muscle proteolysis. FASEB J. 2013; 27(12):5104-11.

Pasiakos SM, Cao JJ, Margolis LM, Sauter ER, Whigham LD, McClung JP, Rood JC, Carbone JW, Combs GF Jr, Young AJ. Effects of high-protein diets on fat-free mass and muscle protein synthesis following weight loss: a randomized controlled trial. FASEB J. 2013; 27(9):3837-47.

Carbone JW, McClung JP, Pasiakos SM. Skeletal muscle responses to negative energy balance: Effects of dietary protein. Adv. Nutr. 2012; 3:119-126.

Lunn WR, Pasiakos SM, Colletto MR, Karfonta KE, Carbone JW, Anderson JM, Rodriguez NR. Chocolate milk and endurance exercise recovery: protein balance, glycogen, and performance. Med Sci Sports Exerc. 2012; 44(4):682-91.

Pasiakos SM, Vislocky LM, Carbone JW, Altieri N, Konopelski K, Freake HC, Anderson JM, Ferrando AA, Wolfe RR, Rodriguez NR. Acute energy deprivation affects skeletal muscle protein synthesis and associated intracellular signaling proteins in physically active adults. J Nutr. 2010. 140(4): 745-51.

Abstracts; oral/poster presentations

Carbone JW, Margolis LM, Berryman CE, Carrigan CT, Murphy NE, Ferrando AA, Young AJ, Pasiakos SM. Muscle proteolysis is largely unaffected by severe energy deficit during high-altitude acclimatization. Curr Dev Nutr. 2018; 2(11):P10-020. [abstract, poster]

Pasiakos SM, Margolis LM, Berryman CE, Carrigan CT, Murphy NE, Carbone JW, Bamman MM, Ferrando AA, Young AJ. Muscle Inflammation Is Upregulated during Energy Deficit at High Altitude and Associated with Increased Myogenesis and Decreased Fat-Free Mass Loss. Curr Dev Nutr. 2018; 2(11):OR42-03. [abstract, oral]

Margolis LM, Berryman CE, Murphy NE, Carrigan CT, Young AJ, Carbone JW, Pasiakos SM. Protein and carbohydrate supplementation modulate skeletal muscle microRNA following aerobic exercise. Curr Dev Nutr. 2018; 2(11):OR09-05. [abstract, oral]

Margolis LM, Berryman CE, Carbone JW, Carrigan CT, Murphy NE, Ferrando AA, Young AJ, Pasiakos SM. Anabolic signaling responses to exercise and recovery whey protein are suppressed at high altitude. FASEB J. April 2018:32(1S):909.6. [abstract, poster]

Ramsey JL, Burley J, Levine S, Carbone JW, Moore RW.  Bilateral Deficit: A Comparison Of Maximal Strength Between The Bilateral And Unilateral Leg Press Exercise. Med Sci Sports Exerc. 2018; 50(5S):666-667. [abstract, poster]

Burley J, Ramsey JL, Levine S, Carbone JW, Moore RW.  Using vertical shoulder press to find occurrences of the bilateral deficit in recreationally trained participants. Med Sci Sports Exerc. 2018; 50(5S):410. [abstract, poster]

Moelter S, Carbone JW, Kret LA, Rainville AJ. Dietetic Internship Directors’ and Preceptors’ Views on Preceptor Recruitment and Retention. J Acad Nutr Diet. 2017; 117(9) Suppl 1: A-53. [abstract, poster]

Berryman CE, Karl JP,  Cole RE,  Kenefick RW,  Margolis LM,  Carbone JW,  Ferrando AA,  Lieberman HR,  Young  AJ,  Pasiakos SM.  Prolonged high altitude exposure exacerbates fat-free mass and fat mass loss during negative energy balance regardless of dietary protein intake. FASEB J. 2017; 31:841.17. [abstract, poster]

Carbone JW, Braun EM, Kraker KA, Percy SA, Fassezke BL, Briolat KN. Preliminary Assessment of a Raspberry Ketone Supplement as a Weight-Loss Aid. FASEB J. 2016; 30:1165.1. [abstract, poster]

Carbone JW, Margolis LM, McClung JP, Cao JJ, Young AJ, Pasiakos SM. Energy intake modulates intramuscular mTORC1 signaling and ubiquitin-mediated proteolysis during energy deficit independent of dietary protein variations. FASEB J. 2013; 27:631.1. [abstract, poster]

Mouch EM, Walz AM, Tomaj L, Carbone JW. A randomized, crossover comparison of the effects of high fructose corn syrup versus sucrose intake on basic physiologic measures. FASEB J. 2013; 27:1074.7. [abstract, poster]

Carbone JW, Margolis LM, Cao JJ, McClung JP, Young AJ, Pasiakos SM. Acute nutrient consumption attenuates intramuscular 26S proteasome activity during energy deficit. FASEB J. 2012; 26:lb299. [abstract, poster]

Carbone JW, Pasiakos SM, Vislocky LM, Anderson JM, Rodriguez NR.  Moderate energy deprivation increases caspase-3 activity and fractional breakdown rate in human skeletal muscle. FASEB J. 2010. 24:740.19. [abstract, poster]

Lunn WR, Colletto MR, Karfonta KE, Anderson JM, Carbone JW, Rodriguez NR. Chocolate milk consumption during recovery from endurance exercise affects intracellular events of skeletal muscle protein synthesis and proteolysis. FASEB J. 2010. 24:97.1. [abstract, oral presentation]

Carbone JW, Pasiakos SM, Vislocky LM, Anderson JM, Giardina C, Bruno RS, Rodriguez NR. Moderate energy deprivation increases ubiquitin proteasome activity in physically active adults following endurance exercise. FASEB J. 2009; 23:720.1. [abstract, poster]

Pasiakos SM, Vislocky LM, Carbone JW, Freake HC, Anderson JM, Ferrando AA, Wolfe RR, Rodriguez NR. Energy-induced changes in skeletal muscle protein synthesis and molecular signaling in physically active adults. FASEB J. 2009; 23:541.2. [abstract,poster]

Vislocky LM, Pasiakos SM, Carbone JW, Freake HC, Anderson JM, Ferrando AA, Wolfe RR, Rodriguez NR. Post-exercise skeletal muscle protein synthesis and intracellular signals during negative energy balance in active adults. Med Sci Sports Exerc. 2009; 41(5):Suppl. [abstract, oral presentation]

Pasiakos SM, Konopelski KL, Vislocky LM, Carbone JW, Altieri NA, Anderson JM, Rodriguez, NR. The effect of acute energy deprivation on metabolic measures during exercise and at rest in physically active adults. Med Sci  Sports Exerc. 2008; 40(5):S472. [abstract, poster]

Pesola JM, Jahns CJ, Carbone JW, Kramer MF, Coen DM. Late genes display unexpected RNA expression profiles in the presence of acyclovir during reactivation of HSV-1 from latency. 2007; International Herpesvirus Workshop, Asheville, NC. [abstract, oral presentation]

Additional Information

Classes Taught

Dr. Carbone teaches a variety of courses within the Dietetics & Human Nutrition program, including Nutrition for Health Professionals (DTC203), Sports Nutrition (DTC204), Macronutrient Metabolism (DTC302), Research Design & Methods (DTC500), and Advanced Topics in Nutrition (DTC618).

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